Open AccessInvestigation of Dielectric Barriers Discharge Plasma Jets for Bactericidal in Chronic Wounds
Author(s) -
Pipath Poramapijitwat,
Phuthidhorn Thana,
Dheerawan Boonyawan,
Keratiya Janpong,
S. Sarapirom
Publication year - 2018
Publication title -
walailak journal of science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.146
H-Index - 15
eISSN - 2228-835X
pISSN - 1686-3933
DOI - 10.48048/wjst.2019.4785
Subject(s) - plasma , dielectric barrier discharge , chemistry , radical , staphylococcus aureus , bacteria , atmospheric pressure plasma , microbiology and biotechnology , biochemistry , biology , physics , quantum mechanics , genetics
The atmospheric pressure plasma technique has been recognized in health care for disinfection in wounds as well as that it can enhance wound healing and reduce pain in patient without side effects. In this study, Dielectric Barrier Discharge Plasma Jets (DBDJs) were used for bactericidal in vitro as well as the efficiency of bacteria killing were investigated using gram positive bacteria, Staphylococcus Aureus (S. Aureus). The DBDJs plasma used He gas at flow rate at 1 L/min, pulse repetition rate between 50 to 110 Hz and exposure time 15 to 60 s for bactericidal. The studies of DBDJs utilized an Optical Emission Spectroscopy (OES) to identify radical species in the plasma. The results of the OES studies showed in DBDJs plasma N2, NO, He and OH radical groups were found. These radicals in plasma played an important role in bactericidal, including wound healing. The intensity of radical in plasma depends on the repetition rate applied by the plasma system. After DBDJs plasma exposure, plates were incubated at 37 °C. Repetition rate and time of plasma exposure were drastically reduced. With the increase in the repetition rate over 100 Hz or exposure time up to 60 s for bactericidal, the reduction of bacteria was increased up to 100 %. The large clear zone showed the efficiency of bacteria killed ability of the plasma.