Open AccessProtective Effects of Thunbergia laurifolia (Linn.) on Organophosphorous (chlorpyrifos)-Induced Cholinesterase Dysfunction
Author(s) -
Malatee Tayeh,
Jirapat Nawarat,
Moe Pwint Phyu,
Jitbanjong Tangpong
Publication year - 2017
Publication title -
walailak journal of science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.146
H-Index - 15
eISSN - 2228-835X
pISSN - 1686-3933
DOI - 10.48048/wjst.2018.4291
Subject(s) - antioxidant , cholinesterase , chemistry , hydrogen peroxide , hydroxyl radical , scavenging , biochemistry , ic50 , in vitro , enzyme , food science , pharmacology , biology
Orgnophosphorous (chyloryrifos) has been widely used for pest control. It is considered to be functionally irreversible inhibitor of cholinesterase (ChE), since the time necessary to liberate the enzyme from inhibition may be in excess of time required for the synthesis of new ChE. This study was designed to investigate the effects of total phenolic content, total antioxidant capacity and free radical scavenging activities in cell free system present in Thunbergia laurifolia (Linn.) (TL) and further examined for their ability against chloripyrifos induced ChE dysfunction in vitro (blood and brain of mice) studies. The results showed that TL has the highest phenolic content, which is significant positive correlation with its antioxidant activity (R2=0.99). Besides, the fifty percent of inhibitory concentration of TL on scavenging free radical activities (IC50) for hydroxyl, superoxide and hydrogen peroxide radical in cell free system were 1.47±0.02, 2.23±0.08 and 1.66±0.09 mg/mL, respectively. We found TL showed a potential inhibition of chloripyrifos-induced ChE dysfunction in a concentration dependent manner. These data suggest that TL exhibits an anti-oxidant and at least in part neuroprotective properties against chloripyrifos-induced ChE alteration. We strongly believe that these antioxidant constituents are prospective novel nutriceuticals.