Fabrication of Sponges from Amnion Hydrogel to Apply as Wound Dressing

A burn is a type of injury to the skin or other tissues. It can give rise to skin defects or even death. In this study, sponges derived from amnion hydrogel were generated to apply as a wound dressing. The sponges were created by combining crosslinking and freeze-drying methods. There were three types of the obtained sponges: MGA-0 (only washed with PBS), MGA-1 (washed in glycine 1 % for one day) and MGA-2 (washed in glycine 1 % for two days). These sponges were evaluated by scanning electron microscope (SEM), mechanical test, swelling, and cytotoxicity. The tensile strength of the sponges was about 1.8 MPa, and the absorption increased during 24 h. The relative growth rates (%RGR) of MGA-0, MGA-1, and MGA-2 were 88.8, 58.2 and 67.2 %, respectively. The obtained results suggested that the MGA-0 sponge has potential for wound dressing application.HIGHLIGHTSThe sponges have been manufactured from human amnion hydrogel by combining crosslinking and freeze-drying methodsThrough the scanning electron microscope (SEM), the surface of the obtained sponges showed a fibrous-like structureThese sponges have good absorbency, the tensile strength of the sponges is about 1.8 MPa like the tensile strength of human skin, and the MGA-0 sponge is not cytotoxicGRAPHICAL ABSTRACT