Open AccessStandardization of surface sterilization protocols for culture establishment in banana cv. Karpurachakkarakeli(AAB) male flower budsin vitro
Author(s) -
Aparna Roy,
M Viswanath,
Chetanchidambar N Mangalore,
Satyabrata Nanda
Publication year - 2021
Publication title -
international journal for innovative engineering and management research
Language(s) - English
Resource type - Journals
ISSN - 2456-5083
DOI - 10.48047/ijiemr/v10/i09/34
Subject(s) - sodium hypochlorite , explant culture , sterilization (economics) , biology , horticulture , cultivar , tissue culture , plant tissue culture , cut flowers , micropropagation , completely randomized design , factorial experiment , organogenesis , botany , in vitro , chemistry , mathematics , biochemistry , statistics , organic chemistry , gene , economics , monetary economics , foreign exchange market , foreign exchange
Plant tissue culture is a proven technique for producing banana seeds in large quantities,uniformly and in a short time to support good quality banana seeds. The banana flower meristemcan be a potential explant. The banana flower meristem offers the opportunity to regenerateplants with agronomic characteristics. This study aimed to regenerate banana flowers in vitrowith different sucrose and BA (Benzyladenine) concentrations after standardized surfacesterilization protocols. The study used a Completely Randomized Design (CRD), two factorialdesignwith surface sterilents and gelling agents. The results showed that the treatmentT15(Sodium hypochlorite (1%) + HgCl2 (0.1%)) in G1 (0.25% gelrite) recorded the lowest fungaland bacterial contamination (0.00, 0.00) & (0.73, 0.53) respectively, in in vitro cultures of maleflower buds of banana cultivar KarpuraChakkarakeli (AAB). While, the combination of BA (4mgL-1) and sucrose (30 mgL-1) concentration had directly induced organogenesis in banana maleflower explants.