Open AccessIn vitro analysis of Punica granatum against bacillary dysentery causing microbes
Author(s) -
Devendra Singh,
Virendra Singh,
Vishnu Agarwal
Publication year - 2020
Publication title -
international journal for innovative engineering and management research
Language(s) - English
Resource type - Journals
ISSN - 2456-5083
DOI - 10.48047/ijiemr/v09/i12/99
Subject(s) - punica , shigella flexneri , bacillary dysentery , microbiology and biotechnology , salmonella enterica , antibacterial activity , salmonella , chemistry , traditional medicine , shigella , biology , bacteria , escherichia coli , dysentery , medicine , biochemistry , genetics , gene
Continuous researches on the herbal drug as an alternative that can solve the problem of increasingthe number of resistant bacterial strains are very important. This research work aims to explore the effectivenessof the Punica granatum L. aqueous extract against the bacterial strain of Shigella flexneri, Salmonella enterica,and E.coli. The MIC was calculated by using the broth dilution method, and the anti-bacterial activity is assessedby using agar well diffusion assay. The bactericidal activity was examined using the broth dilution method andthe cytotoxicity was analyzed using the brine shrimp test. The obtained results show that the aqueous extractsof P. granatum showed antibacterial activity against bacillary dysentery, causing microbes, and were found nontoxicin brine shrimp lethality assay. The aqueous extracts of P. granatum also show the bactericidal effect andanti-bacterial effects against the bacterial strain of Shigella flexneri, Salmonella enterica, and E.coli, which areresponsible for causing bacillary dysentery. The obtained evidence shows that the aqueous extracts of P.granatum have anti-bacillary activity.