Open AccessPhytochemical Analysis and Antibacterial Activity of Purple Leaf Extract [Graptophyllum pictum (L.) Griff] Against Streptococcus mutans
Author(s) -
Devi Eka Juniarti,
Tuti Kusumaningsih,
Wisnu Setyari Juliastuti,
Adioro Soetojo,
Nabilla Dara Wungsu
Publication year - 2021
Publication title -
acta medica philippina/acta medica philippina
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.128
H-Index - 4
eISSN - 2094-9278
pISSN - 0001-6071
DOI - 10.47895/amp.v55i8.2125
Subject(s) - streptococcus mutans , minimum bactericidal concentration , minimum inhibitory concentration , phytochemical , tannin , saponin , antibacterial activity , food science , chemistry , traditional medicine , petri dish , antimicrobial , microbiology and biotechnology , biology , botany , bacteria , medicine , genetics , alternative medicine , pathology
Background. Streptococcus mutans is the leading cause of dental caries. One of many medicinal plants, purple leaf [Graptophyllum pictum (L.) Griff], which contains flavonoids, alkaloids, tannins, steroids, and saponins, is a potential antibacterial agent.
Objective. This study aimed to determine the antibacterial activity of purple leaf extract (Graptophyllum pictum L. Griff) against Streptococcus mutans.
Methods. Streptococcus mutans were suspended in several Graptophyllum pictum (L.) Griff extract concentrations in a BHIB medium using the dilution method so that the concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78% were obtained. Each tube was incubated for 24 hours, then subcultured in a Tryptone Yeast Extract Cystine medium in a petri dish using a spreader. Each petri dish was set for 24 hours; the growth of the colony, using CFU/mL unit, was manually calculated. The samples were then subjected to microbiological analysis. The Tukey's Honest Significant Difference test was performed to determine if the relationship between the sets of data in the treatment group is statistically significant (p<0.05).
Results. Purple leaf extract contains bioactive compounds such as flavonoid, alkaloid, tannin, triterpenoid/ steroid, and saponin. The Minimum Inhibitory Concentration (MIC) of Graptophyllum pictum (L.) Griff against Streptococcus mutans was in concentration 3.125%, and the Minimum Bactericidal Concentration (MBC) was in concentration 6.25%.
Conclusion. Purple Leaf Extract [Graptophyllum pictum (L.) Griff] has antibacterial activity against Streptococcus mutans.