Open AccessT45G Adiponectin Gene Polymorphism and its Association with Hyperglycemia in Adult Filipinos Seen at the Philippine General Hospital – A Pilot Study
Author(s) -
Elizabeth Paz-Pacheco,
Eva Maria C. Cutiongco-de la Paz,
Angelique Bea Uy
Publication year - 2020
Publication title -
acta medica philippina/acta medica philippina
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.128
H-Index - 4
eISSN - 2094-9278
pISSN - 0001-6071
DOI - 10.47895/amp.v54i4.1918
Subject(s) - adiponectin , single nucleotide polymorphism , medicine , snp , diabetes mellitus , endocrinology , insulin resistance , type 2 diabetes , insulin , polymorphism (computer science) , outpatient clinic , genotype , gastroenterology , gene , biology , genetics
. Adiponectin is an adipocytokine known to have anti-inflammatory and anti-atherogenic effects. It appears to impact insulin resistance and the subsequent development of type 2 diabetes mellitus (T2D). The gene encoding adiponectin ADIPOQ, has single nucleotide polymorphisms (SNPs) that can be useful biomarkers to predict development of T2D; with the T/G polymorphism of SNP +45 in exon 2 being the most common.Objective. This study was conducted to evaluate the association of T45G adiponectin gene polymorphism with hyperglycemia among adult Filipinos seen at the outpatient department of the Philippine General Hospital.Methods. This is a matched case-control study, with duration of 12 months. DNA was extracted using the QIAGEN MIDI Blood Extraction Kit. The genomic DNA obtained was then subjected to real time PCR for SNP detection.Results. One hundred (100) adults were enrolled; forty-three (43) had normoglycemia, while fifty seven (57) had hyperglycemia, after a 75-g oral glucose tolerance test. Hyperglycemic subjects were older (44±15.6 years vs. 52±8.3 years, p-value 0.002), and had lower HDL levels (58.5±16.0 mg/dLvs. 47.8+11.8 mg/dL, p-value 0.000). Among thirty-nine (39) participants found to have the T45G adiponectin gene polymorphism, 22 or 56.4% were hyperglycemic while 17 or 43.6% were normoglycemic.Conclusion. There was no significant association observed between the T45G SNP and presence of hyperglycemia.