Open AccessSimultaneous determination of steviol glycosides in stevia by high performance liquid chromatography
Author(s) -
Huyền Trang Lưu Thị,
Thanh Hương Đoàn Thị,
Trang Vũ Thị,
Sơn Trần Cao,
Ánh Hường Nguyễn Thị,
M.L.N. Thi
Publication year - 2019
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.93
Subject(s) - steviol , stevioside , rebaudioside a , stevia rebaudiana , stevia , chromatography , glycoside , high performance liquid chromatography , chemistry , methanol , food science , organic chemistry , medicine , alternative medicine , pathology
A method for determination of steviol glucosides in stevia using high performance liquid chromatography was developed and validated. The compounds were extracted from the matrices with methanol for 60 minutes, cleaned by Oasis HLB column and then determined by HPLC using C18 column (250 mm x 4.6 mm x 5 µm) and PDA detector. The calibration curves were linear in range of 1 to 100 μg/mL; the RSD was of 1.39 ­ 2.85%; and the recovery was of 93 ­ 105%. The method was applied to analyze 09 stevia samples collected from markets in Hanoi (including dry stevia rebaudiana, stevia powder). The results showed that composition of steviol glycosides differs from sample to sample. Besides, stevioside and rebaudioside A are the most abundant steviol glycosides in the samples.