Open AccessDetermination of sibutramine and phenolphthalein in weightloss tea, coffee, and milk by ultra-performance liquid chromatography mass spectrometry UPLC-MS/MS
Author(s) -
Thân Trương Văn,
Duy Nguyễn Thành,
Kiệt Lý Tuấn,
Vinh Hoàng Ngọc,
Hải Chu Vân,
Experimentation Ho Chi Minh City
Publication year - 2019
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.88
Subject(s) - chromatography , chemistry , quechers , sibutramine , high performance liquid chromatography , phenolphthalein , formic acid , distilled water , mass spectrometry , liquid chromatography–mass spectrometry , weight loss , pesticide residue , medicine , obesity , pesticide , agronomy , biology
A rapid, simply and effective method using ultra­performance liquid chromatography coupled to mass spectrometry (MS) detection was developed in order to determine sibutramine and phenolphthalein in weight­loss tea, coffee, and milk. QuEChERS technique was used for cleaning­up sample matrices. The extracts were separated on C18 LC columns using the gradient elution of 0.1% formic acid in distilled water and acetonitrile. The recoveries at the concentration of 50 µg/kg were from 90.1 to 105.8% (with RSDR of 4.04 ­ 4.24%), from 91.9 to 100.2% (with RSDR of 3.20 ­ 4.71%), and from 86.5 to 100.5% (with RSDR of 5.17 ­ 5.49%) for weight­loss tea, coffee, and milk, respectively. Method quantitation limits were of 30 µg/kg.