Open AccessDetermination of sialic acid in dietary supplement by liquid chromatography tandem-mass spectrometry (LC-MS/MS)
Author(s) -
Hồng Ngọc Nguyễn Thị,
Thanh Hoa Mạc Thị,
Mai Hoa Dương Thị,
Sơn Trần Hùng,
Khánh Cao Công,
Thanh Hà Phạm Thị,
Hồng Hảo Lê Thị
Publication year - 2019
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.87
Subject(s) - neuraminic acid , chromatography , chemistry , formic acid , sialic acid , liquid chromatography–mass spectrometry , tandem mass spectrometry , selected reaction monitoring , mass spectrometry , high performance liquid chromatography , repeatability , biochemistry
Simultaneous determination of sialic acids (N-acetyl neuraminic acid - NANA and N-glycolyl neuraminic acid - NGNA) in dietary supplement matrices by liquid chromatography tandem-mass spectrometry (LC-MS/MS) had been developed. Sialic acids were separated by using a C18 column (75 mm x 3.9 mm, 2.5 µm) and isocratic mobile phase of acid formic 0.1 %: acetonitrile (80:20, v/v). Sialic acids were totally hydrolyzed by formic acid 1M at 80oC in 2 hours before being analyzed by LC-MS/MS. It was proved that the method with the linearity range of 0.04 - 4.25 µg/ml had highly specificity, selectivity, repeatability, and recovery rate, that well satisfied AOAC requirements. The method has been applied to detect sialic acids in commercial dietary supplements.