Open AccessDetermination of rutin content in sophora japonia L. Extracts used as raw material in supplements by high performance liquid chromatography
Author(s) -
Đạt Nguyễn Thành,
Hồng Hạnh Nguyễn Thị,
Thu Đàm Thị,
Thúy Chu Thị,
Nương Hoàng Thị
Publication year - 2019
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.80
Subject(s) - rutin , chromatography , high performance liquid chromatography , sophora , chemistry , methanol , acetic acid , extraction (chemistry) , ethanol , antioxidant , biochemistry , medicine , alternative medicine , organic chemistry , traditional chinese medicine , pathology
An HPLC-UV method was proposed in order to determine Rutin in Sophora japonica L. extract samples (3 samples extracted with 30% ethanol, 3 samples extracted with 70% ethanol and 3 samples extracted with water). The chromatographic conditions were: Agilent Zorbax Eclipse XDB C18 Column (150 x 4.6 mm, 5 µm); UV-Vis detector (257 nm); mobile phase: a mixture of methanol and 1% acetic acid solution (40:60, v/v). The linear range was of 4.97 - 298.47µg/mL; the LOQ was of 0.205 µg/mL and the accuracy was within 99.87% - 102.3%. The method was proved to be suitable for the determination of Rutin in Sophora japonica L. extracts. Rutin content in samples varied from 30.14% to 38.67%.