Open AccessSimultaneous screening of 5 allergens in food by using liquid chromatography triple quadrupole mass spectrometry
Author(s) -
Ha Binh Nguyen Thi,
Thu Nguyen Thi,
Ngoc Lan Dang Thi,
Hai Nguyen Thi,
Sơn Trần Cao
Publication year - 2019
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.70
Subject(s) - chromatography , chemistry , electrospray ionization , mass spectrometry , selected reaction monitoring , detection limit , tandem mass spectrometry , triple quadrupole mass spectrometer , extraction (chemistry) , trypsin , allergen , liquid chromatography–mass spectrometry , enzyme , biochemistry , biology , allergy , immunology
The liquid chromatography tandem mass spectrometry with electrospray ionization (ESI) source in multiple reactions monitoring (MRM) mode has been used to detect five allergen including milk, egg, peanut, soyabean, and walnut in milk, dairy products, and confectionery. The allergenic proteins from food matrices were extracted with an extraction buffer (50 mM of TRIS- saline, 2 M of urea, and 25 mM of DTT) and then enzymatically digested with trypsin to form peptides. The peptides were eventually detected on a LC-MS/MS Triple Quad 5500 system from AB SCIEX. As a result, each allergen was characterized by a corresponding specific peptide. The limit of detection was of 3 µg/g for milk, 5 µg/g for peanut, 10 µg/g for soyabean and walnut and 20 µg/g for egg.