Open AccessDetermination of Phytosterols in vegetable oils by GC-MS method
Author(s) -
Hiền Đặng Thu,
Ngọc Anh Mai Thị,
Hồng Ngọc Nguyễn Thị
Publication year - 2020
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.1759
Subject(s) - chromatography , derivatization , chemistry , gas chromatography–mass spectrometry , detection limit , mass spectrometry , gas chromatography , trimethylsilyl , extraction (chemistry) , toluene , bstfa , phytosterol , organic chemistry
In this study, a method for determination of six phytosterols by gas chromatography-mass spectrometry with derivatization in vegetable oils was validated. The samples were hydrolyzed in an alkaline media at 70°C for 60 min. Then, the samples were performed liquid-liquid extraction with toluene. The phytosterols are derivatized to trimethylsilyl ethers and then analyzed by gas chromatography-mass spectrometry. The limit of detection and limit of quantification was 5 and 15 mg/kg, respectively. Recoveries of six phytosterols were between 93.5% and 101%.