Open AccessScreenning and determination of polyhydroxyalkanoate production by Bacillus sp. strains
Author(s) -
Đà Nguyễn Thị,
Linh Nguyễn Trọng,
Trang Nguyen Thi Thu,
Hải Trần Mạnh,
Huyền Lã Thị
Publication year - 2020
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.1724
Subject(s) - polyhydroxyalkanoates , bacillus megaterium , bacteria , bacillus (shape) , 16s ribosomal rna , microbiology and biotechnology , strain (injury) , biology , food science , biochemistry , chemistry , genetics , anatomy
Polyhydroxyalkanoates (PHAs) are biodegradable polymers produced by microbes as reserve material when they were cultured in a limitation of nutrients and excess C source mediums. PHAs can be synthesized by various bacteria including gram positive and gram-negative bacteria. Among the PHA-producing gram-positive bacteria, Bacillus sp. produce and accumulate various monomer compositions of PHAs. In this study, about 83,3% of strains (05/06 strains) can accumulate PHAs production. Bacillus sp. DV01 accumulated a amount of PHA 23,9% of CWD after 96h when cultured in modified basal medium containing 1% (w/v) glucose. Bacillus sp. DV01 was identified by sequecing the gene coding for 16S rRNA. BLAST results showed the obtained 16S rRNA sequence of this strain revealed 100% with the Bacillus megaterium and so-called Bacillus megaterium DV01.