Open AccessResearch for toxicity and determination IC50 of tea leaf extracts on cervical cancer cell and breast cancer cell MCF7 by in vitro
Author(s) -
Vân Anh Nguyễn Thị,
Thanh Nguyen Duc
Publication year - 2020
Publication title -
kiểm nghiệm và an toàn thực phẩm/kiểm nghiệm và an toàn thực phẩm (online)
Language(s) - English
Resource type - Journals
eISSN - 2734-9152
pISSN - 2615-9252
DOI - 10.47866/2615-9252/vjfc.109
Subject(s) - hela , chromatography , sephadex , elution , chemistry , toxicity , ic50 , cell culture , in vitro , cervical carcinoma , extraction (chemistry) , cancer , cervical cancer , biology , biochemistry , medicine , organic chemistry , genetics , enzyme
After extracting tea leaves with methanol 80% (acidified with HCl), the extraction was purified through two phases: the first stage used the column of silica gel with gradient elution, the second stage continued to be isolated on Sephadex LH-20 column with isometric elution mode, K powder was obtained. The test of toxicity of K powder on two MCF37 breast cancer cell lines and Hela cervical cancer was carried out and obtained the following results: IC50 of the composition with the line MCF37 was 31.62 µg/mL and with the line Hela was 50.1 µg/mL. Compared with the positive control group of Taxol showed that the efficacy of the obtained product was lower. However, this is a new direction in the use of drugs derived from plants in general and tea leaves in particular.