Open AccessReview on Microbial Sources, Production, Purification and Potential Industrial Applications of Laccases
Author(s) -
Amit Rastogi,
Rajesh Kumar Singh,
Ahmed Barhoums
Publication year - 2021
Publication title -
international journal of advances in agricultural science and technology
Language(s) - English
Resource type - Journals
ISSN - 2348-1358
DOI - 10.47856/ijaast.2021.v08i12.004
Subject(s) - laccase , chemistry , solid state fermentation , fermentation , polyphenol oxidase , metal ions in aqueous solution , multicopper oxidase , pulp and paper industry , wastewater , metal , organic chemistry , environmental science , environmental engineering , peroxidase , enzyme , engineering
Laccase (EC 1.10.3.2) is a multicopper blue oxidase which are involved in the oxidation of a broad range of organic substrates, including phenols, polyphenols, anilines, and even certain inorganic compounds by a one-electron transfer mechanism. Laccases are widely distributed in bacteria, fungai, insects and higher plants. There are mainly two production techniques for cultivation of laccase such as submersed fermentation and solid- state fermentation. This paper briefly discuss the effect of carbon source, effect of nitrogen source, effect of inducers, effects of surfactants, effect of agitator, influence of metal ions and use of agro-industrial waste in production medium. The paper also discussed the purification techniques such as ammonium sulphate precipitation for extraction purpose followed by dialysis and ion-exchange chromatography as well characterization techniques. Laccases are known to show application ranging from pharmaceutical industries to textile sector as well as in biosensor development.