Open AccessPectinase Production from Banana Peel Biomass via the Optimization of the Solid-state Fermentation Conditions of Aspergillus niger Strain
Author(s) -
Nazaitulshila Rasit,
Yong Sin Sze,
Mohd Ali Hassan,
Ooi Chee Kuan,
Sofiah Hamzah,
Wan Rafizah Wan Abdullah@Wan Abd. Rahman,
Md. Nurul Islam Siddique
Publication year - 2021
Publication title -
pertanika journal of science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.174
H-Index - 15
eISSN - 2231-8526
pISSN - 0128-7680
DOI - 10.47836/pjst.30.1.14
Subject(s) - solid state fermentation , pectinase , aspergillus niger , fermentation , food science , biomass (ecology) , factorial experiment , bran , spore , chemistry , botany , banana peel , horticulture , raw material , mathematics , agronomy , biology , biochemistry , enzyme , statistics , organic chemistry
In this study, the biomass of banana peel was used to produce pectinase via optimization of solid-state fermentation conditions of the filamentous fungi Aspergillus nigeA. niger). The operating conditions of solid-state fermentation were optimized using the method of full factorial design with incubation temperature ranging between 25 °C and 35 °C, moisture content between 40% and 60%, and inoculum size between 1.6 x 106 spores/mL and 1.4 x 107 spores/mL. Optimizing the solid-state fermentation conditions appeared crucial to minimize the sample used in this experimental design and determine the significant correlation between the operating conditions. A relatively high maximal pectinase production of 27 UmL-1 was attained at 35° C of incubation, 60% of moisture content, and 1.6 x 106 spores/mL of inoculum size with a relatively low amount of substrate (5 g). Given that the production of pectinase with other substrates (e.g., pineapple waste, lemon peel, cassava waste, and wheat bran) generally ranges between 3 U/mL and 16 U/mL (Abdullah et al., 2018; Handa et al., 2016; Melnichuk et al., 2020; Thangaratham and Manimegalai, 2014; Salim et al., 2017), thus the yield of pectinase derived from the banana peel in this study (27 U/mL) was considered moderately high. The findings of this study indicated that the biomass of banana peel would be a potential substrate for pectinase production via the solid-state fermentation of A. niger.