Open AccessEFFECT OF DIFFERENT FREEZING PERIODS OF SERUM SAMPLES ONCOMMON BIOCHEMICAL TESTS
Author(s) -
Alea Farhan SALMAN,
Huda Ibraheem ABD-AL-LATTEF,
Mushtaq Mufleh KHAZEEM
Publication year - 2021
Language(s) - English
Resource type - Conference proceedings
DOI - 10.47832/minarcongress3-6
Subject(s) - lactate dehydrogenase , uric acid , albumin , alkaline phosphatase , chemistry , urea , calcium , chromatography , serum albumin , biochemistry , enzyme , organic chemistry
Blood samples are often collected, stored for certain periods and analyzed in the laboratory, and this affects the results of the analysis. The aim of this study was to determine the analytical persistence of serum samples expose to storage temperature range and times prior to analysis. Materials and methods: Serum samples were obtained from 36 persons. After measuring the fresh sample, the rest of the serum sample was divided into 5 groups and stored at -20 ˚C. A group of sera was kept frozen for up to 42 days then analyzed for stability. A total of 9 chemistry analytics were assayed(glucose, urea, uric acid, total protein, albumin, calcium, lactate dehydrogenase (LDH), and alkaline phosphatase) at each time point. The results were compared with those obtained from the initial analysis of fresh samples. Results: Among the analytics studied, cholesterol was stable in all conditions. Glucose, urea, uric acid, total protein, albumin, calcium, lactate dehydrogenase (LDH), and alkaline phosphatase were changed significantly (P < 0.005). Conclusions: These results can be used to determine which chemical analysis gives incorrect results when exposed to different storage times but some of the chemical analysis was not affected by the delay and storage conditions for up to 6 weeks prior to analysis. Key words: Serum, Stability, Storage Time, Storage Temperature Range.