Open AccessInvitro germination of Bael (Aegle marmelos (L.) Corr.) seeds for clonal propagation
Author(s) -
K. Indhumathi
Publication year - 2020
Publication title -
annals of plant and soil research/annals of plant and soil research
Language(s) - English
Resource type - Journals
eISSN - 2347-6036
pISSN - 0972-1959
DOI - 10.47815/apsr.2020.10020
Subject(s) - germination , seedling , coat , explant culture , shoot , biology , horticulture , murashige and skoog medium , in vitro , botany , bark (sound) , paleontology , ecology , biochemistry
Aegle marmelos, commonly known as Bael, is one of the most important medicinal plants of Indian tradition. All parts of the tree viz. leaf, fruit, bark and root have medicinal properties and have been used in many traditional medicine systems. The present study trial has been taken up at Horticultural College & Research Institute, Coimbatore to establish a protocol for in vitro germination of seeds to use it further for clonal propagation. The investigation revealed that the seeds without seed coat gave more number of multiple shoots (5.57) where as the seeds with seed coat gave single seedling. The days taken for germination was lesser in seeds without seed coat (14.76 days) than the the seeds with seed coat (20.59 days). The longest seedling was observed in the seeds with seed coat (2.09 cm) compared to that of seeds without seed coat (1.22 cm). the days taken for budbreak, number of shoots were higher in the MS medium supplemented with BAP 1.5 mgl-1. Comparison between the explants from in vitro and field grown seedlings showed that the time taken for culture response was much earlier in the in vitro derived seedlings. All the explants showed better response in the basal MS medium supplemented with BAP 1.5 mgl-1