Open AccessMethod Development and Validation of Salmeterol xinofoate by HPLC
Author(s) -
Sahebrao H. Shembade,
Sagar S. Landage,
Ashapak M. Tamboli,
Ritesh S. Bhate,
Kaustubh Gavali,
Lingraj V. Sargar
Publication year - 2021
Publication title -
international journal of pharmaceutical sciences and medicine
Language(s) - English
Resource type - Journals
ISSN - 2519-9889
DOI - 10.47760/ijpsm.2021.v06i06.006
Subject(s) - salmeterol , chromatography , high performance liquid chromatography , formic acid , chemistry , acetonitrile , analytical chemistry (journal) , medicine , copd , psychiatry
A rapid and precise high performance liquid chromatography method has been developed for the validation of Salmeterol xinofoate in its pure dosage form. The separation was carried out on Agilent Zorbax Bonus RP- (250mm ×4.6mm 5μ) column with a mobile phase consisting of 0.1% Formic acid: Acetonitrile in the ratio of 64:36 v/v as a mobile phase and flow rate is 1ml/min. The detection was carried out at wavelength 234nm. The column thermostatically controlled at 30℃. The retention time of Salmeterol was found to be 1.96 min. The Salmeterol xinofoate followed linearity in the concentration range of 40-60μg/mL with r2= 0.999. The developed method was validated for sensitivity, accuracy and precision. The sample was scanned from 200- 400nm with PDA detector. The % recovery of sample was found to be. The LOD and LOQ of the Salmeterol xinofoate was found to be 2.67μg/ml and 8.08μg/ml respectively. The suitability of this HPLC method for quantitative estimation of Salmeterol xinofoate was proved by validation by the requirements of ICH guidelines.