Open AccessA Novel Method Development and Validation or the Estimation of Meloxicam in Bulk and Dosage Forms by RP – UFLC
Author(s) -
Kranthi Yalla,
Kotte Raju,
Gowtham Reddy Cheruku,
Charan Raj Koutharapu,
Ghouse Mohiuddin Mohammad,
Divya M.S.L
Publication year - 2022
Publication title -
international journal of pharmaceutical sciences review and research
Language(s) - English
Resource type - Journals
ISSN - 0976-044X
DOI - 10.47583/ijpsrr.2022.v73i01.023
Subject(s) - meloxicam , chromatography , ammonium acetate , chemistry , volumetric flow rate , elution , acetic acid , dosage form , acetonitrile , high performance liquid chromatography , pharmacology , medicine , biochemistry , physics , quantum mechanics
Meloxicam is a non-steroidal anti-inflammatory drug and selective inhibitor of COX-2, it is used particularly for the management of rheumatoid arthritis. In this RP-HPLC method chromatographic separation was achieved on Isocratic elution of the mobile phase Acetonitrile: Ammonium acetate: methanol: glacial acetic acid (Proportion of mobile phase-45:40:10:5 %v/v/v/v) with the flow rate of 1 ml/min. Separation was performed on C18 column (150 mm × 4.6 mm inner diameter, 5 μm particle size). The flow rate was 1.0 ml/min and detector wavelength were kept at 363 nm for monitoring the separation. The method was developed and validated. The linearity range was found to be 10 - 50μg/ml. Regression coefficient was found to be 0.995. Precision study showed % RSD values are less than 2% in all selected concentrations. The recovery studies for Meloxicam were found to be in the range of 99.7 – 100.2%. System suitability parameters remain unchanged even if the composition of mobile phase and wavelengths were changed. The proposed method was very simple, precise, accurate and rapid for determination of Meloxicam from pure and its dosage forms.