Open AccessEvaluation of Anti-Urolithiatic Activity of Ethanolic Extract of Delonix Regia Leaves in Experimental Animal
Author(s) -
Sonali S. Nipate,
Rajanigandha R Jori
Publication year - 2022
Publication title -
international journal of pharmaceutical sciences review and research
Language(s) - English
Resource type - Journals
ISSN - 0976-044X
DOI - 10.47583/ijpsrr.2022.v72i02.012
Subject(s) - ammonium chloride , chemistry , ethylene glycol , calcium oxalate , aqua regia , chromatography , urine , biochemistry , organic chemistry , metal
The aim of the present study was to investigate the anti-urolithiatic effect of Delonix regia leaves in ethylene glycol and ammonium chloride induced urolithiasis in wistar rats. Renal stone was induced in animals by 0.75% ethylene glycol and 1 % ammonium chloride in drinking water for 28 days. In this study the test groups animals were administered with ethanolic extract of D.Regia at doses 200mg/kg and 400mg/kg once in day from 15th day to 28th day. Cystone (75mg/kg) was used as standard drug. In this study in vitro crystallization is done by using nucleation assay and aggregation assay by using calcium chloride and sodium oxalate. The effect of Delonix regia on various physical and biochemical parameters in urine as well as serum was evaluated. The present study, results reveals that the administration of 400mg/kg dose of Delonix regia reduced the biochemical parameter than 200mg/kg and states that higher dose is an effective dose for treatment of urolithiasis.