Open AccessGreen Synthesis of Silver Nanoparticles Using Stem of Ruta graveolens L: Antibacterial, Cytotoxic and Antioxidant Activity
Author(s) -
Remya Varadarajan,
Bhakti Hirani,
Chandravilas Kesavan Prashant,
Krutika Devidas Patil,
Rakhi Amarchandra Jana,
Shamal Hrushikesh Chavan
Publication year - 2022
Publication title -
international journal of pharmaceutical sciences review and research
Language(s) - English
Resource type - Journals
ISSN - 0976-044X
DOI - 10.47583/ijpsrr.2022.v72i01.002
Subject(s) - ruta graveolens , silver nanoparticle , chemistry , antioxidant , zeta potential , antibacterial activity , nuclear chemistry , abts , mtt assay , cytotoxicity , nanoparticle , terpenoid , nanotechnology , stereochemistry , apoptosis , in vitro , dpph , organic chemistry , biochemistry , botany , biology , materials science , bacteria , genetics
The green chemistry approach using plants involves phytoconstituent mediated reduction process for synthesis of nanoparticles.Biogenic Silver nanoparticles (AgNPs) were synthesized using aqueous stem extract of Ruta graveolens. The adopted biologicalmethod is compatible with green chemistry principles because the plant extract acts as a dual functional molecule: as reductant anda stabilizing agent for the synthesis of AgNPs. The UV–visible spectra confirmed that reduction of silver ions for Ruta stem extract at485 nm on 24-hour incubation. FT-IR spectroscopic spectrum showed the peaks at 3507, 2829, 1579, 1010, 755 which arecharacteristic of flavonoids and terpenoids present in the stem. The zeta potential of Ruta AgNPs was found to be -35.4 mV whichindicate that the nanoparticles are quite stable due to charge accumulation on its surface. The TEM micrograph reveals that theparticles were of spherical shape & monodispersed, the average size of the particles is around 15 nm. Ruta AgNPs showed cytotoxicityagainst cancer cells and the results indicated that with the increase in Ruta AgNPs concentration there was a significant (p < 0.05)decrease in cell viability of HepG2 as compared to control. The antioxidant activity of Ruta AgNP was confirmed by ABTS radicalscavenging activity. Ruta AgNPs may provide novel resources for the evaluation and development of newer, safer, and effectiveantimicrobial and antiproliferative drug formulations.