Open AccessScreening, Isolation and Biochemical Characterization of Laccase Producing Bacteria from Contaminated Sites
Author(s) -
Siva Jyothi J.,
Kishore Kumar K,
Hema Latha E.
Publication year - 2021
Publication title -
international journal of pharmaceutical sciences review and research
Language(s) - English
Resource type - Journals
ISSN - 0976-044X
DOI - 10.47583/ijpsrr.2021.v71i02.018
Subject(s) - guaiacol , laccase , nutrient agar , bacteria , biology , isolation (microbiology) , microbiology and biotechnology , lignin , enterobacter , agar plate , food science , bacillus (shape) , agar , botany , escherichia coli , biochemistry , enzyme , genetics , gene
Screening and isolation of Laccase producing bacteria from Guntur District soil was carried out to assess the diversity of Lignocellulosedegrading bacteria. Isolation and identification of environmental friendly bacteria for lignin degradation becomes an essential one,because all the researchers are mainly concentrating on fungal strains. However, bacteria seem to play a leading role in decomposinglignin. For isolation of Laccase producing bacteria nutrient agar medium containing guaiacol was used. Total nine bacterial strainswere isolated from soil samples collected from different regions of Guntur district. Preliminary screening of bacterial strains wascarried out on guaiacol containing nutrient agar medium for laccase production. Formation of green colour using ABTS (2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) confirms the capability of laccase production by the bacterial strains. Nine bacterialstrains showed positive results. High laccase producing bacterial isolates were examined for morphological and biochemicalcharacteristics according to Bergey’s Manual of Systematic Bacteriology. The predominant isolates were identified as Bacillus and Enterobacter species.