Open AccessApoptosis Inducing Activity of Diospyros melanoxylon Bark Extract on Molt-3 Cell Line via Activation of Mitochondrial Membrane Caspase Cascade and Cell Cycle Arrest with its Antioxidant Potential
Author(s) -
Nilanjana Deb,
Tarun Jha,
Snehasikta Swarnakar,
Shila Elizabeth Besra
Publication year - 2021
Publication title -
international journal of pharmaceutical sciences review and research
Language(s) - English
Resource type - Journals
ISSN - 0976-044X
DOI - 10.47583/ijpsrr.2021.v71i01.018
Subject(s) - apoptosis , propidium iodide , cytochrome c , microbiology and biotechnology , chemistry , mitochondrion , programmed cell death , cell culture , annexin , biology , biochemistry , genetics
The present work aimed to investigate the apoptosis activity of Diospyros melanoxylon of bark extract (DMBE) on Molt-3 humanacute T lymphoblastic leukemia cell line & RAW 264.7 murine macrophage cell lysate respectively. Cell growth inhibition was observedby using MTT assay. DMBE significantly decreased cell count and caused apoptosis, as confirmed by light, fluorescence, confocalmicroscopy and by flow-cytometric analysis using Annexin-V FITC and propidium iodide (PI). MOLT-3 cells showed DMBE induced cellcycle arrest, and apoptosis was mediated through mitochondrial intrinsic pathway involving the release of cytochrome c into thecytosol and activation of caspase-9 and caspase-3. DMBE induced changes in cellular ROS production upon stimulation with DCFDA.