Open AccessReview on Analytical Methods for Determination of Lamivudine, Dolutegravir and Tenofovir Disoproxil Fumarate in Fixed Dose Combination
Author(s) -
Y. Haribabu,
K. Nihila,
Vk. Sheeja,
M. B. Akhil
Publication year - 2021
Publication title -
international journal of pharmaceutical sciences review and research
Language(s) - English
Resource type - Journals
ISSN - 0976-044X
DOI - 10.47583/ijpsrr.2021.v71i01.003
Subject(s) - dolutegravir , lamivudine , dosage form , chromatography , high performance liquid chromatography , chemistry , human immunodeficiency virus (hiv) , medicine , antiretroviral therapy , viral load , virology , virus , hepatitis b virus
Pharmaceutical medicines play an important role in human life that helps to cure different diseases. For the chemical andpharmaceutical analysis of the drug effective quality control and pharmacodynamic and pharmacokinetic studies are needed. Severalmethods have been developed and validated for its pharmaceutical and biological materials since it was introduced as an importantantiretroviral agent. The literature survey reveals that only four RP HPLC method and one HPTLC was developed for the simultaneousestimation of Dolutegravir, Lamivudine and Tenofovir disoproxil fumarate in tablet dosage form. These three drugs are used asantiretroviral medicines which are used for HIV or AIDS prevention and treatment. The goal of this review is to define and establish asimple, precise and selective method for estimating the dosage of Lamivudine, Tenofovir Disoproxil fumarate and Dolutegravir inbiological and pharmaceutical dosage form using the HPLC, HPTLC, UPLC, UV Visible spectroscopy, LC/MS, Infrared spectroscopy,NMR spectroscopy, Microbiological assay, Electrochemical studies and Capillary electrophoresis. UV-detector HPLC is commonly usedin pharmaceuticals and LC-MS are widely used for biological materials with mass and tandem mass spectrometer detector systems.Various parameters such as device suitability, process accuracy, precision, linearity, detection limit will validate the UV Visiblespectroscopy and RP-HPLC technique.