Correlation of Direct LDL Versus Friedewald’s, Modified Friedewald’s and Anandraja’s Formula for Estimating LDL Cholesterol Levels along with LDH and Troponin I in Coronary Artery Disease Patients

The objective of the study is to compare the Friedewald formula, Modified Friedewald formula, and Anandaraja formula with directhomogeneous assay for low- density lipoprotein cholesterol (LDL-C) levels in CAD patients. Study Design is Cross-sectional study.Healthy subjects of both the gender, aged 18 - 75 years were included in this study. Lipid profile, LDH and Troponin I of both thecontrols and patients sample were collected from OPD of Central Hospital laboratory of Gayatri Vidhya Parishad Institute of HealthCare and Medical Technology, Visakhapatnam. LDL-C estimation was done by direct homogenous assay and also calculated by usingthe Friedewald’s Formula, Modified Friedewald’s Formula and Anandaraja’s Formula for assessing and validity of the LDL cholesterol.LDH were measured kinetically UV method by Chem Ultra reagent system Pack and Cardiac Troponin I by JusCheck Rapid card test.One hundred samples were analyzed in this study, Out of that 50 are Known CAD patient samples and 50 are controls. The comparisonof Lipid profile of Variable versus group. The P value also > 0.05 statistically significant. The Mean value for LDH in Case (Patients) is486.2, whereas for control, it was 328.3. It shows highly significant for this parameter. The association between Case (Patients) andControl for Troponin I was highly significant in this study. In our study, we conclude that, modified friedewald’s formula was closureto the direct method. The performance of calculated methods was not uniform at different TG levels. Even though, ModifiedFriedewald’s formula is closure to Direct LDL, For laboratory setup, Novel and Innovative direct homogeneous assays are most reliableand accurate for the analysis of LDL cholesterol.