Open AccessHigh Performance Thin Layer Chromatography Fingerprinting Studies of Croton klotzschianus (Wight) Thw.
Author(s) -
Jerad Suresh,
Saravana Ganthi A
Publication year - 2021
Publication title -
international journal of pharmaceutical sciences review and research
Language(s) - English
Resource type - Journals
ISSN - 0976-044X
DOI - 10.47583/ijpsrr.2021.v69i02.017
Subject(s) - high performance thin layer chromatography , chromatography , phytochemical , chemistry , thin layer chromatography , formic acid , ethyl acetate , fingerprint (computing) , croton , traditional medicine , organic chemistry , medicine , biochemistry , computer security , computer science
Most of the traditional medicinal plants in India are not scientifically validated. Scientific evaluation along with traditional knowledgeis essential to obtain effective drugs for commercial purpose. The present study was aimed at High Performance Thin LayerChromatography (HPTLC) analysis of ethanol extracts of leaf, stem and root of Croton klotzschianus. The chemical fingerprint throughHPTLC Studies was carried out as per the standard method. The HPTLC shows ethanolic extracts of Croton klotzschianus at UV short and long wavelength 254 and 366 using Hexane: Ethyl acetate: Toluene: Chloroform: Methanol: Formic acid (4:2.5:1.5:0.8:1:0.2) as mobile phase and the Rf values were recorded. The HPTLC fingerprint profile of leaf, stem and root extracts was exhibits 12 peaks each. The results of qualitative phytochemical screening confirm the presence of flavonoids. The HPTLC analysis to help in proper identification and quantification of marker compounds.