Open AccessIsolation, Characterization and Antibacterial Activity of ECDY Steroid from Roots of Polycarpaea corymbosa LAM
Author(s) -
I. Carolin Nimila,
K. G. Lalitha,
R.Sambath kumar
Publication year - 2021
Publication title -
international journal of pharmaceutical sciences review and research
Language(s) - English
Resource type - Journals
ISSN - 0976-044X
DOI - 10.47583/ijpsrr.2021.v68i02.014
Subject(s) - dept , petroleum ether , ethyl acetate , chemistry , steroid , extraction (chemistry) , chromatography , antibacterial activity , traditional medicine , benzene , stereochemistry , organic chemistry , biology , bacteria , biochemistry , hormone , medicine , genetics
A new phytoecdysteroid (2- deoxy 20 hydroxy 22 cinnamoyl ecdysone 3, 25 diacetate) was isolated from the roots of Polycarpaea corymbosa Lam (family: caryophyllaceae).The powdered roots were extracted with petroleum ether ( 60-80? C), benzene, ethyl acetate and ethyl alcohol in soxhlet apparatus by simultaneous extraction. The highest yield of benzene extract was chosen for the isolation of ecdysteroid. The white solid pure compound was isolated and tested by TLC and respond positively for Liebermann - burchard reaction. Their structure has been established by UV, IR, 1H, 13C-NMR DEPT -90, DEPT -135 and EI- MS experiments. All the extracts and isolated compound were screened for anti-bacterial activity against B.subtilis, P.vulgaris, E.coli, P.aeruginosa by Kirbybauer method.