Open AccessIn vitro Anti-Arthritic activity of Pseudarthria viscida against Protein Denaturation and Proteinase enzyme
Author(s) -
P. Saravana Pandian
Publication year - 2020
Publication title -
international journal of ayurvedic medicine
Language(s) - English
Resource type - Journals
ISSN - 0976-5921
DOI - 10.47552/ijam.v11i2.1532
Subject(s) - diclofenac sodium , ethanol , chemistry , in vitro , enzyme , denaturation (fissile materials) , aqueous solution , enzyme assay , solvent , arthritis , chromatography , pharmacology , biochemistry , medicine , nuclear chemistry
Arthritis is an autoimmune with chronic inflammatory, the patient has very painful due deformities and bone erosion which is caused by damage of the joints. The plant Pseudarthria viscida was collected from the Thirunelveli district and extracted with aqueous and ethanol solvent. The two method was used for determination of invitro anti-arthritic activity. The Inhibition of Protein Denaturation Method shows the anti-arthritic activity with the value from 40.46±0.72 to 78.36±0.64 for aqueous extract and 48.62±0.86 to 84.42±0.86 for ethanol extract and Inhibition of Proteinase Enzyme Activity shows 38.62±0.32 to 72.58±0.58 in aqueous extract and 46.28±0.58 to 80.52±0.56 in ethanol extract. Diclofenac sodium were used as standard, the concentration is 100, 200, 300, 400, and 500. In both the method the concentration of 500Microgram per milliliters shows maximum inhibition and compare to both extract the ethanol shows better activity than aqueous extract.