Open AccessA Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus Samples
Author(s) -
Mateen Izhar,
Asma Akram,
Anwaar Basheer,
Mariya Ali,
Asma Yaqoob,
Asim Saifullah
Publication year - 2020
Publication title -
proceedings
Language(s) - English
Resource type - Journals
eISSN - 2518-203X
pISSN - 1815-4905
DOI - 10.47489/p000s343z7571-5mc
Subject(s) - staphylococcus aureus , microbiology and biotechnology , gentamicin , antibiotic sensitivity , fusidic acid , antimicrobial , ciprofloxacin , sccmec , erythromycin , antibiotic resistance , agar diffusion test , biology , antibiotics , methicillin resistant staphylococcus aureus , medicine , bacteria , genetics
Staphylococcus aureus harboring Panton Valentine Leucocidin gene are emerging and spreading worldwide. PVL gene was first identified by Noel Panton and Francis Valentine in 1932 who IC Pakistan only limited data is available regarding the effect of PVL gene on sensitivity pattern of Staphylococcus aureus. Therefore, this study was conducted to understand the antimicrobial sensitivity pattern of both PVL positive and negative Staphylococcus aureus isolates.Aims & Objectives: This study was conducted to understand the antimicrobial sensitivity pattern of both PVL positive and PVL negative Staphylococcus aureus isolated from pus samples received from various indoor and outdoor departments of a tertiary care hospital of Lahore.Place and duration of study: Microbiology and Molecular Biology Laboratory Shaikh Zayed Hospital Lahore. Duration of study is one year after the approval of research topic.Material & Methods: A total of 384 Staphylococcus aureus isolates from skin and soft tissue infections were identified and selected. Their antimicrobial sensitivity testing was done by Kirby disc diffusion method using Muller Hinton agar.Results: Frequencies of PVL gene in MRSA and MSSA were 51% and 44% respectively. Frequency of PVL gene was also found to be high in Ciprofloxacin sensitive, Gentamicin sensitive, Erythromycin resistant and fusidic acid resistant isolates.Conclusion: Almost half of Staphylococcus aureus isolates were found PVL positive. They were mostly multidrug resistant. The PVL positive Staphylococcus aureus isolates showed high resistance against antibiotics than PVL negative isolates