Open AccessPotentials of Acremonium butyri fungus in pre-treatment and hydrolysis using Rice husk substrate for biofuel production: A short communication
Author(s) -
Azlin Sanusi
Publication year - 2020
Publication title -
umyu journal of microbiology research
Language(s) - English
Resource type - Journals
ISSN - 2616-0668
DOI - 10.47430/ujmr.2052.018
Subject(s) - husk , lignin , hemicellulose , acremonium , cellulose , chemistry , sugar , food science , distilled water , hydrolysis , biofuel , pulp and paper industry , substrate (aquarium) , botany , biology , organic chemistry , microbiology and biotechnology , chromatography , ecology , engineering
There is increasing interest in the area of biofuel production due to fear of fossil fuel extinction as a result of over exploitation and crises. During biofuel production, substrate needs to undergo pre-treatment and hydrolysis where acids and alkali are mostly used. In this study, Acremonium butyri was used for both pre-treatment and hydrolysis. Structural compositions of the rice husk were determined. Extractives, hemicellulose and Lignin content was determined via extraction using Soxhlet extractor while cellulose was determined as the difference from the extractives, hemicelluloses and lignin. Acremonium butyri was isolated from dried roots of Piliostigma reticulatum (Kalgo) by keeping the roots in a clean plastic for a period of 7 days on moist environment after which fungal growth appeared. The growth was aseptically transferred on to prepared S.D.A plate and kept at room temperature. The fungal growth was identified as based on the physical and microscopic characteristics. About 50g of rice husk was mixed with 500 ml of distilled water in the ration of 1:10 to obtain homogenous slurry and then inoculated with 2ml of prepared Acremonium butyri solution and incubated at room temperature for up to 3 weeks with frequent shaking at certain intervals. Reducing sugar test was carried out to determine the reducing sugar released with UV-VIS spectrophotometer. The results obtained indicate that rice husk contained 32%, 30%, 29% and 8.4% of cellulose, hemicelluloses, extractives and lignin respectively. And a total of 0.936g/l of reducing sugar was released after 3 weeks of pre-treatment. The results implies that Acremonium butyri separated the component of rice husk (pre-treatment) as well as break down cellulose and hemicelluloses into its monomers (hydrolysis) thereby releasing sugar. Hence, Acremonium butyri is a good microorganism for biological pre-tretment and hydrolysis. Keywords: Acremonium butyri, Rice Husk, Pre-treatment, Hydrolysis, Biofuel