Open AccessInfluence of biotechnological parameters on the yield of macrostructures from unfertilized seed germs of diploid sugar beet
Author(s) -
O. A. Zinchenko,
N. S. Zatserkovna,
Olena Ukrainets,
А. В. Заболотна
Publication year - 2021
Publication title -
naukovì pracì ìnstitutu bìoenergetičnih kulʹtur ta cukrovih burâkìv
Language(s) - English
Resource type - Journals
ISSN - 2410-1281
DOI - 10.47414/np.29.2021.249934
Subject(s) - sterility , sugar beet , sodium hypochlorite , biology , sugar , sterilization (economics) , ploidy , horticulture , yield (engineering) , nutrient , callus , botany , chemistry , food science , ecology , biochemistry , materials science , organic chemistry , gene , metallurgy , monetary economics , economics , foreign exchange market , foreign exchange
Purpose. To determine the influence of biotechnological parameters on the yield of macrostructures from unfertilized seed germs of diploid sugar beet.
Methods. Biotechnological, laboratory, analytical, statistical.
Results. It was found that the use of 35% sodium hypochlorite solution at an exposure of 40 min allows to obtain from 73.13 to 75.83% of sterile seed germs. Exposure of 50 min allows to obtain the sterility of the source material from 83.58 to 85.39%. Sterilization of explants for 60 min allows to obtain sterility of the source material from 86.88 to 92.80%. The share of infected seed germs with increasing exposure decreased from 20.09–22.14 to 6.52–12.61%. The yield of macrostructures has been experimentally confirmed to significantly depend on breeding genotype and type of medium. The largest number of calluses (10–80%) was formed with the use of the Hamburg and Eveleg’s medium. With the use of he Murasige and Skoog’s medium, their share was 10–35%. Noteworthy, in breeding genotypes 07–181, 80% of genotypes formed buds and 35% formed calluses in the Hamburg and Eveleg’s medium. Of breeding genotypes 07–178, 55% of genotypes formed a callus and 80% buds.
Conclusions. As a result of the conducted researches the influence of biotechnological parameters (exposure to 35% solution of sodium hypochlorite, type of nutrient medium) on the yield of macrostructures from unfertilized seed germs of diploid sugar beet was determined. It is optimal to carry out treatment with 35% sodium hypochlorite solution for 50–60 minutes, regardless of the selection number of sugar beet. To obtain macrostructures from unfertilized seed germs of diploid sugar beet, it is necessary to use the Hamburg and Eveleg’s medium for breeding genotypes 07–188, 07–178 and 07–181.