Assessment of Interleukin_10 level in Sudanese Patients with Chronic Lymphocytic Leukemia in Khartoum State

Background: Chronic lymphocytic leukemia (CLL) is the most predominant leukemia among adults. Interleukin‑10 (IL‑10) secretion has potent immunosuppressive effects and it has a strong stimulating impact on B-lymphocytes, inducing proliferation and differentiation. This study was conducted to investigate the serum levels of IL-10 in patients with CLL (cases) and comparing them with apparently healthy age and gender match volunteers as control group. Method: A total of 88 blood samples were randomly collected. 44 blood samples were withdrawn from cases, while the rest were obtained from control. Serum samples after centrifugation of blood were obtained for IL-10 level evaluation. Human IL-10 ELISA max Deluxe kit was used to determine IL-10 in each sample following manufacture instructions. The Data was analyzed through statistical package for the social science (SPSS) version 20. Results: Our results showed that the mean of IL_10 level among CLL patients (22.7±5.34 pg\ml) was significantly higher (P-value: 0.002) than the mean of IL_10 level among control group (4.99±0.55 pg\ml). Among case groups females showed IL-10 mean of 29.09±10.85 pg/ml that was significantly higher than male’s 18.22±5.04 pg/ml with p-value 0.023. Our result finds no significant difference between cases below 55years (23.62±8.03 pg/ml) and above55 years (22.07±7.19 pg/ml) in the level of IL-10 with P-value 0.893. Conclusion: In conclusion, this study determined higher serum IL-10 levels in CLL patients compared with healthy controls. Gender and duration of disease have significant effect on serum IL-10 level among CLL cases. However, sample size in this study was small and larger scale research is needed in this field with the inclusion of several factors such as disease, grade and outcome.