Open AccessNew Method for Isolation of DNA from River Sediment Microbes of Sungai Kelantan, Malaysia
Author(s) -
Noratikah Kamal Baharin,
Noorkhalilie Che Abd Aziz,
Mohamad Faisal,
Suganthi Appalasamy
Publication year - 2017
Publication title -
journal of tropical resources and sustainable science/journal of tropical resources and sustainable science
Language(s) - English
Resource type - Journals
eISSN - 2462-2389
pISSN - 2289-3946
DOI - 10.47253/jtrss.v5i2.590
Subject(s) - dna extraction , extraction (chemistry) , genomic dna , dna , sediment , yield (engineering) , isolation (microbiology) , environmental science , chromatography , biology , environmental chemistry , chemistry , microbiology and biotechnology , polymerase chain reaction , gene , genetics , materials science , paleontology , metallurgy
Extraction of pure genomic DNA from microbes isolated from river sediments often hindered byco-isolated carryovers from the reagents used which reduces the yield and quality of DNA.Existing methods were lengthy, expensive and does not result in high yield of genomic DNA inshort time. This study describes a rapid and inexpensive DNA extraction method that involvedminimal purification step without phenol or other carcinogenic reagents. The microbial DNAextraction steps also does not require the use of special laboratory equipment. This method usesCetyltrimethylammonium bromide (CTAB) followed by ethanol based purification steps andwithin one hour the extraction of microbial DNA could be completed. The extracted genomicDNA from microbes isolated from river sediment of Sungai Kelantan, Malaysia using the methoddescribed in this study is of quality suitable for other downstream researches such asmetagenomics sequencing.