Open AccessPROCESS OPTIMIZATION USING QUALITY BY DESIGN (QBD) APPROACH OF A GENTAMICIN LOADED PLGA BIOCOMPOSITE
Author(s) -
Maria-Viorica Ciocîlteu,
Oana Eleicolaescu,
Andreea Gabriela Mocanu,
Claudiu Nicolicescu,
Gabriela Rău,
Johny Neamţu,
Emilia Amzoiu,
Carmen Nicoleta Oancea,
Adina Turcu-Ştiolică
Publication year - 2021
Publication title -
journal of science and arts
Language(s) - English
Resource type - Journals
eISSN - 2068-3049
pISSN - 1844-9581
DOI - 10.46939/j.sci.arts-21.4-b02
Subject(s) - plga , gentamicin , biocomposite , nanoparticle , materials science , biomedical engineering , bioburden , chemical engineering , chemistry , nanotechnology , composite material , surgery , composite number , antibiotics , medicine , biochemistry , engineering
Osteomyelitis continues to be a major concern when orthopedic surgery is performed. Orthopedic infections have an incidence of 5% to 10% but their management proves to be quite difficult due to both biofilm formation and limited access of the drug to the infected area when systemic treatment is employed. The aim of the study was to optimize the synthesis process of a gentamicin loaded poly(-lactic-co-glycolic) acid (PLGA) based biodegradable composite by varying parameters that affect both efficiency encapsulation and nanoparticle size. Furthermore, a kinetic study was conducted to study the biodegradation process of the polymer. Gentamicin loaded PLGA nanoparticles were obtained using the double emulsion technique which allows the variation of several factors such as gentamycin concentration, PLGA concentration, buffer concentration and stirring speed. Out of the four factors evaluated, gentamicin concentration had the highest impact on both encapsulation efficiency and nanoparticle size. A few relevant interactions between factors were also registered.