Open AccessANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA
Author(s) -
Ratih Widyasari,
AUTHOR_ID,
Willy Hadinata Halim,
Atia Nurul Sidiqa,
Dewa Made Wedagama,
AUTHOR_ID,
AUTHOR_ID,
AUTHOR_ID
Publication year - 2021
Publication title -
interdental jurnal kedokteran gigi (ijkg)/interdental jurnal kedokteran gigi
Language(s) - English
Resource type - Journals
eISSN - 2685-5208
pISSN - 1979-9144
DOI - 10.46862/interdental.v17i2.2400
Subject(s) - enterococcus faecalis , lime , agar diffusion test , agar , bacteria , chemistry , traditional medicine , antibacterial activity , agar plate , food science , biology , medicine , paleontology , genetics , staphylococcus aureus
Endodontic treatment failure can be caused by the bacterium E. faecalis, which has a prevalence of up to 90%. E. faecalis is difficult to eradicate because it forms a biofilm to defend itself against antibacterial agents. Lime leaves are one of the many herbal products available inIndonesia (Citrus hystrix DC). Lime leaf extracts include antibacterialcomponents such as essential oils, phenolics, alkaloids, saponins, andtannins. The purpose of this study was to investigate the antibacterial activity of lime leaf extract against E. faecalis bacterium. Material and Methods:The agar diffusion technique was used for four experimental groups, using lime leaf extract at 4% (group 1), 8% (group 2), and 16% (group 3) as the negative control. Each group was repeated six times on Muller Hinton Agar (MHA) medium with a paper disc, and the diameter of the inhibitory zone was measured with a caliper. Results and Discussions: The results revealed that a 4 % concentration of lime leaf extract had an average inhibition zone diameter of 7.31 mm, an 8 % concentration had an average inhibition zone diameter of 8.59 mm, and a 16 % concentration had an average inhibition zone diameter of 11.41 mm. The difference in the inhibition zone wasstatistically significant (p= 0,000) evaluated using the one-way ANOVA. Conclusion: It can be concluded that lime leaf extract is antibacterialpotential against E. faecalis bacteria in the endodontic treatment.