Open AccessVALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEED
Author(s) -
Sandra Jakšić,
Željko Mihaljev,
Milica Živkov Baloš,
Žoran Mašić
Publication year - 2009
Publication title -
arhiv veterinarske medicine/archives of veterinary medicine
Language(s) - English
Resource type - Journals
eISSN - 2683-4138
pISSN - 1820-9955
DOI - 10.46784/e-avm.v2i2.250
Subject(s) - kjeldahl method , combustion , detection limit , chemistry , standard deviation , chromatography , nitrogen , mathematics , statistics , organic chemistry
Crude proteins content is one of the most important parameters of animal feed quality. For total proteins determination Kjeldahl method is the most known and most used. However, total combustion method, well-known as Dumas, is also recognized as standard method. In this paper the model of Dumas validation for total combustion method on the instrument Elementar- Rapid N is shown. The validation involves determination of linearity, detection limit, accuracy and precision, as the standard required. In validation procedure standard substances were used and the results were processed with suitable statistics methods in Microsoft Excel. Examined validation parameters show that the tested method can be used for accurate determination of total nitrogen (protein) determination in animal feed samples.