DETERMINATION OF Bisphenol A RELEASED FROM POLYCARBONATE INFANT FEEDING BOTTLES BY UV-VIS SPECTROPHOTOMETRY

Bisphenol A (BPA) is a synthetic organic compound used as a raw material for the production of polycarbonate plastics, which are widely used in infant feeding bottles, kitchen utensils and other food packaging materials. The migration of bisphenol A from polycarbonate feeding bottles into water and milk samples was investigated using UV-Vis spectrophotometry based on diazotization-coupling reaction. The proposed method involves diazotization of 4-aminobenzenesulphonic acid under acidic condition in the presence of sodium nitrite at low temperature, followed by its coupling with Bisphenol A, in alkaline medium to produce yellow color azo-dye. The diazo–coupling reagents and time were optimized, the azo-dye formed has absorption maximum at 439nm. BPA was detected within the range of 0.37 – 5.93 µg/mL in the samples using standard addition method. The validation parameters were LOD: 0.48 µg/mL, LOQ: 1.62 µg/mL, while recovery for spiked samples were averagely 97.87 – 99.63 % and %RSD ranged between 0.12- 4.9%. Milk samples have exceeded the tolerable daily intake (TDI) for BPA of 50μg/kgbw/day recommended by EFSA.