Open AccessCiprofloxacin loaded castor oil based emulsion: Its antimicrobial, hematotoxicity and soft tissue pathophysiological study in Wister rats
Author(s) -
A. Bamisaye,
C. O. Eromosele,
E. O. Dare,
O. A. Akinloye
Publication year - 2020
Publication title -
bulletin of the chemical society of nigeria
Language(s) - English
Resource type - Journals
ISSN - 0795-2066
DOI - 10.46602/jcsn.v45i5.531
Subject(s) - ciprofloxacin , pharmacology , antimicrobial , spleen , potency , minimum inhibitory concentration , chemistry , minimum bactericidal concentration , distilled water , medicine , biology , microbiology and biotechnology , antibiotics , biochemistry , chromatography , in vitro
The emergence of nanocarrier for drug delivery has been flanked with great achievements in the field of nanomedicine. It is however unsafe to assume their toxicological safety. This necessitated examining the toxico-dynamics of ciprofloxacin loaded castor oil based emulsion (COAB+Cp). The emulsions were prepared by mechano-chemical process. The effects of 10 mL per kg of body-weight (BW) of COAB+Cp was estimated in Adult Wistar rats (n=10) for 21 days vis-à-vis a control set up, treated with equal volume of distilled water. The biochemical and haematological parameters were conducted with histopathology of the heart, kidney, spleen and liver. Also, the antimicrobial study on Bacillus. Subtilis (BS) was carried out using the agar well diffusion method. No observable abnormal change in BW gain of both test and control animals. Treatment of animals with COAB+Cp did not cause any observable significant change at P > 0.05 in the blood-chemistry parameters and hematological indices. The photopathological examinations indicates that the histological architecture of vital organs (heart, liver, kidney and spleen) was not compromised in COAB+Cp treated animals. While the recorded value of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of COAB+Cp corroborated its mean zone of inhibition (ZI) values, signifying a better bioactivity of COAB+Cp compared to Cp on BS. COAB+Cp has no cardiotoxic, hematotoxic and hepatotoxic effects on Wistar rats, with increased potency of the encapsulated Cp thus appears promising as a safe vehicle for oral delivery of ciprofloxacin.