Open AccessIMMUNE PROPERTIES IN THE PRODUCTED RECOMBINANT SIP YOUNG 6 HIS PROTEIN FROM THE SEA STAR IGKAPPA GENE THROUGH HEK 293 EBNA CELLS REVEALING AGAINST SPIKE-RBD PROTEIN AND HRP ONE CHANGES IN SEA STAR GENE EXPRESSION?
Publication year - 2021
Publication title -
american international journal of biology and life sciences
Language(s) - English
Resource type - Journals
eISSN - 2641-5623
pISSN - 2641-5615
DOI - 10.46545/aijbls.v3i1.309
Subject(s) - hek 293 cells , cloning (programming) , recombinant dna , microbiology and biotechnology , gene , biology , molecular cloning , chemistry , gene expression , genetics , computer science , programming language
In 2014 we have isolated and cloned the sea star Igkappa gene which induced an anti-HRP primitive invertebrate antibody. We attempt, in the present work, to produce a SIP Young 6 His protein through HeK 293 EBNA cells after Igkappa gene cloning. This protein bound HRP by the use of Escherichia coli as vector of cloning but not HRP with HeK cells as vector. Is there changes in sea star IGKappa gene expression? We notice also that the SARS-CoV-2-SPIKE RBD Protein didn’t bind to SIP Young 6 His protein when compared to HRP.