Open AccessGreen synthesis of silver nanoparticles from Alpinia galanga extract with microwave irradiation and antibacterial activity against Escherichia coli
Author(s) -
Yuli Haryani,
Yonatha Melanie,
Maria Novita,
. Yuharmen,
Yuaurulita,
Rudi Hendra,
Ganis Fia Kartika
Publication year - 2022
Publication title -
pharmacy education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.198
H-Index - 17
eISSN - 1477-2701
pISSN - 1560-2214
DOI - 10.46542/pe.2022.222.2023
Subject(s) - resazurin , silver nanoparticle , chemistry , nuclear chemistry , silver nitrate , absorbance , antibacterial activity , aqueous solution , escherichia coli , nanoparticle , microwave irradiation , chromatography , nanotechnology , bacteria , organic chemistry , biochemistry , materials science , catalysis , biology , genetics , gene
Background: This study deals with the rapid green synthesis of silver nanoparticles (Ag NPs) by microwave irradiation using water extract of Alpinia galanga as a reducing agent.Methods: The effect of concentrations of water extract and silver nitrate aqueous solution (1 mM) on the synthesis was investigated using the ratios 1:1, 1:5, 1:10, and 1:20. The formation of Ag NPs was observed after being microwaved for 30, 60, 90, 120, 150, and 180 seconds. Results: The best Ag NPs were produced from the ratio of 1:10 after 180 s irradiation, confirmed by the optimum absorbance at 450 nm using a UV-visible spectrophotometer. Transmission electron microscopy analysis showed that the size of the Ag NPs was 50 nm. Their antibacterial activity was determined using Resazurin microtiter assay against Escherichia coli, and the results showed that it inhibited 94.4% of E. coli. It was significantly different (p < 0.05) with the inhibition of extract and Amoxsan, which inhibited 71.1% and 85.8% of E. coli, respectively.Conclusion: The use of microwave irradiation on the synthesis of silver nanoparticles using Alpinia galanga extract as a reducing agent appears to be rapid and eco-friendly.