Open AccessCharacterisation and Biological Activity of Stingray Venom (HimanturaImbricata)
Author(s) -
Ramachandiran Sivaramakrishnan Selvaraj Uthra,
Ganapathy Sivaranjani Thirukumar Sangeshwari,
Shankar Kanchana and Muthuvel Arumugam
Publication year - 2021
Publication title -
international journal of modern trends in science and technology
Language(s) - English
Resource type - Journals
ISSN - 2455-3778
DOI - 10.46501/ijmtst0703006
Subject(s) - partial thromboplastin time , coagulation , fibrin , fibrinogen , prothrombin time , envenomation , chemistry , thrombin time , venom , gel electrophoresis , polyacrylamide gel electrophoresis , stingray , biochemistry , chromatography , biology , medicine , immunology , enzyme , ecology
Stingrays envenomation in humanswere the common accident in the marine and freshwater ecosystem. Todetermine such effect species Himantura imbricata have been used to elucidate Hemolytic activity, PlasmaCoagulation, Fibrin coagulation, Fibrinolytic activity, Activated Partial Thromboplastin Time (APTT),andProthrombin Time (PT) effects were studied. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE), Amino acid analysis by RP-HPLC, FTIR spectral analysis and SEM were carried forcharacterization studies. The results show the of plasma coagulation, fibrin coagulation, of this stingrayvenom delays the coagulation of citrated plasma. APTT and PT results showed intrinsic and extrinsiccoagulation factors that were responsible in time delay when compared with the control. Moreover, thesebiological experiments and characterization studies aided in understanding the envenoming factors andthese results might base the development of treatments for complex diseases.