Open AccessIsolation of Keratinolytic Microorganism Streptomyces chartreusis strain ISP 5085 from the Soil Sample: Characterization, Degradation and Microbial Identification using 16S rDNA Region
Author(s) -
Jitendra Nayak and Bhikhu More
Publication year - 2020
Publication title -
international journal of modern trends in science and technology
Language(s) - English
Resource type - Journals
ISSN - 2455-3778
DOI - 10.46501/ijmtst061013
Subject(s) - microorganism , feather , keratinase , 16s ribosomal rna , food science , biology , strain (injury) , degradation (telecommunications) , feather meal , keratin , bacteria , fish meal , ecology , telecommunications , genetics , anatomy , computer science , paleontology , fishery , fish <actinopterygii>
Keratinolytic microorganisms have a broad significance in feather waste degradation and it can be usedfor advancement of livestock feed and production of protein hydrolysates. Production of keratin into theenvironment in different forms is very high which leads to setback for its degradation. An approach fordegradation of keratin based wastes through potential microorganism with higher efficiency can be attained.The study shows that maximum DFD% i.e. Degree of Feather (keratin) Degradation, through the isolates isachieved, when nutrient medium is kept at temperature 30 °C and pH-8.5 supplemented with dehydratedpowdered form of chicken feathers as keratin source for 72 h – 120 h. Chicken feathers acts as a bettersubstrate and source of keratin content. A molecular approach was necessary therefore; phylogenetic trees ofthe isolate were constructed with neighbour-joining method through the Microbial Identification using 16SrDNA Region, which showed that sequence exhibited a high level of homology with Streptomyces chartreusisstrain ISP 5085. The present study deals with identification of isolates that play a significant role in thedegradation of chicken feather waste as well as keratin degradation ability of the isolates and optimumconditions required for it.