Open AccessOptimization of Cellulase Enzyme Production by Co-cultures of Fungi Isolated from Lignocellulosic Waste
Author(s) -
Francis C. St. John,
P A Soloman
Publication year - 2020
Publication title -
international journal of modern trends in science and technology
Language(s) - English
Resource type - Journals
ISSN - 2455-3778
DOI - 10.46501/ijmtst060504
Subject(s) - cellulase , trichoderma viride , penicillium citrinum , cellulosic ethanol , response surface methodology , cellulose , food science , chemistry , substrate (aquarium) , aspergillus oryzae , hydrolysis , penicillium , incubation , trichoderma , botany , chromatography , biology , biochemistry , fermentation , ecology
Fruit wastes were incubated with the mixture of cellulolytic fungi Penicillium citrinum, Aspergillus oryzae,and Trichoderma viride to hydrolyze the cellulosic components and to increase the degree of degradation. .The batch experiments are statistically designed and performed using Box-Benhken method of ResponseSurface Methodology to investigate the influence of major parameters viz., incubation time, temperature, pH,moisture content and substrate concentration on cellulase enzyme production. Maximum cellulase productionof 2.03 Units/ml (U/ml) was detected by the RSM method in a mixed culture containing fungi at a ratio of 1: 1:1 under optimal conditions at an incubation time of 5.27 days, a temperature of 34.09 °C, pH 4.85, moisturecontent of 63.83% and a substrate concentration of 5.03%.