Open AccessTítulo en español.
Author(s) -
H. E. Waterworth,
R. H. Lawson,
R. P. Kahn
Publication year - 1974
Publication title -
the journal of agriculture of the university of puerto rico/the journal of agriculture of the university of puerto rico
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.123
H-Index - 10
eISSN - 2308-1759
pISSN - 0041-994X
DOI - 10.46429/jaupr.v58i3.10670
Subject(s) - humanities , dioscorea , biology , medicine , philosophy , pathology , alternative medicine
A virus latent in yam species Dioscorea floribunda and D. composita (DLV) was purified by use of powdered activated charcoal and magnesium-treated bentonite to clarify extracted Dioscorea leaf sap. The virus produced a single band in sucrose-density gradients. Antisera produced in a rabbit reached a titer of 1:16,000. Purified DLV did not react with antiscra to any of 25 rod-shaped viruses. Myzus persicae (Sulzer), the green peach aphid, did not transmit DLV from infected Dioscorea plants. Seventy-four percent of the particles measured 395-445 nm. Inoculated D. floribunda seedlings showed no symptoms, but the virus was observed in dip preparations from systemically infected growth. DLV occurred in combination with a second virus that was aphid-transmissible and which incited green banding symptoms.