Open AccessComparison of Four Methods for Isolating Nematodes from Soil Samples
Author(s) -
Alejandro Ayala,
Jessé Román,
A. C. Tarjan
Publication year - 1963
Publication title -
the journal of agriculture of the university of puerto rico/the journal of agriculture of the university of puerto rico
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.123
H-Index - 10
eISSN - 2308-1759
pISSN - 0041-994X
DOI - 10.46429/jaupr.v47i4.12957
Subject(s) - soil water , sugar , petri dish , environmental science , soil science , chemistry , biology , food science , microbiology and biotechnology
Four methods for extracting nematodes from soil samples were investigated, using soils ranging from predominately sand to mostly clay. These were: The Oostenbrink Flotation Apparatus III, a modified Oostenbrink elutriator, a sugar-flotation technique, and a sieving Petri-dish method. It was found that no one method can be regarded as completely satisfactory for all types of soils. The sugar-flotation technique was more effective with sandy soils, while the other three methods were more effective with clay soils. The sugar-flotation technique proved superior to the other methods for recovery of ring nematodes from all the types of soils used.