Open AccessELISA and Fourier-transform infrared spectroscopy
Author(s) -
Alejandro Clavijo-Maldonado,
Ênio Ferreira,
Jorge E. Pérez-Cárdenas,
Carlos Vargas Hernández,
Fredy A. RiveraPáez
Publication year - 2021
Publication title -
veterinarska stanica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.173
H-Index - 4
eISSN - 1849-1170
pISSN - 0350-7149
DOI - 10.46419/vs.53.4.4
Subject(s) - absorbance , receiver operating characteristic , fourier transform infrared spectroscopy , chemistry , area under the curve , infrared spectroscopy , analytical chemistry (journal) , spectroscopy , microbiology and biotechnology , chromatography , biology , medicine , bioinformatics , optics , physics , organic chemistry , quantum mechanics
ELISA and FTIR assay techniques were used to identify HER2 gene expression in the blood serum of female dogs and to characterise the biochemical composition. ELISA tests assess the stage of primary tumour development and evolution, while FTIR allows for a complete characterisation of biomolecules associated with the tumoral process. Blood serum samples from 30 female dogs were analysed. Concentrations of the HER2/neu protein were detected using ELISA kits specific for canine and human detection. Infrared spectroscopy (IR) was conducted in absorbance mode at a frequency range of 400–4000 cm-1 and a resolution of 4 cm-1 over 50 scans. The ELISA cut-off for HER2 protein concentration in blood serum was determined using the receiver operating characteristic (ROC) curve and by estimating the area under the curve (AUC) at a 95% confidence interval (CI=95%). The ROC curves in the canine and human ELISA tests were 0.75 and 0.45, respectively. The representative IR spectra for HER2 gene expression corresponded to lipids (1161 cm-1, 1452 cm-1, 2851 cm-1). This study contributes to the knowledge of HER2 through the identification of biochemical features associated with the changes in the HER2/neu+ and HER2/neu- states.