Open AccessLoop Mediated Isothermal Amplification Technique (LAMP): A rapid Tool For Detection Of mitochondrial Cytochrome b Gene Of Dorcas gazelle
Author(s) -
Reem Rabie Mohammed Salih,
Imadeldin E. Aradaib,
A. E. Karrar,
Haytham Hashim Gibreel
Publication year - 2021
Publication title -
genetics and biodiversity journal
Language(s) - English
Resource type - Journals
eISSN - 2602-5582
pISSN - 2588-185X
DOI - 10.46325/gabj.v4i1.129
Subject(s) - loop mediated isothermal amplification , recombinase polymerase amplification , polymerase chain reaction , mitochondrial dna , nucleic acid , biology , microbiology and biotechnology , cytochrome b , dna , gene , polymerase , genetics
Loop-mediated isothermal amplification (LAMP) assay was introduced in the year 2000 by Notomi, as a highly sensitive, specific and cost-effective technique for microbial identification. In contrast to the polymerase chain reaction (PCR) technology in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is carried out at a constant temperature and does not require a thermal cycler. LAMP, a simple DNA amplification technique, with its field amenable nature has been used to detect a variety of pathogens including viruses, fungi, bacteria and parasites and in most of the cases it surpasses polymerase chain reaction. In this study the authors investigated the Loop mediated isothermal amplification technique (LAMP) which is a novel nucleic acid amplification technique. They tried to apply LAMP technique to detection of mitochondrial cytochrome b gene in dorcas gazelles. They designed LAMP specific primers for targeted gene and have verified the LAMP sensitivity up to 4 particles. The authors suggested that LAMP technique could be an appropriate replacement for PCR and may be useful in low resource or field settings where conventional DNA or RNA extraction prior.