Open AccessIsolation, Production, Purification, Assay and Characterization of Insulin From Goat (Capra Hircus) Pancreas By RDT Using Saccharomyces Cerevisae Vector
Publication year - 2020
Publication title -
journal of science and technolgy
Language(s) - English
Resource type - Journals
ISSN - 2456-5660
DOI - 10.46243/jst.2020.v5.i6.pp107-110
Subject(s) - recombinant dna , insulin , pancreas , capra hircus , biology , biochemistry , microbiology and biotechnology , chemistry , endocrinology , gene , zoology
Insulin is a polypeptide hormone secreted by the β-cell of Islet’s of langerhan’s of the pancreas andcosists of two polypeptide chain-A and chain- B. It is linked by two inter chain disulphide bonds (A7- B7 and A20-B19)and also has an intra-chain disulphide bond in the A-Chain (A6-A11). For production of Insulin from goat pancreassaccharomyces cerevisae using as a vector. In this study Recombinant DNA technology, chromatography,Elecrophoresis, Spectrophotometer, SDS-PAGE, zymography etc techaniques were used. Humulin was tanken as amarker. It was found, goat insulin showed good result. 5.8 – 6.5 KDa recombinant insulin was calculated. It did notshow antigenic proterties.